Reading a DM3 File with ScopeReader

Gerd Duscher

10/09/2020

This document is a simple example of how to read DM3 Files into the sidpy dataformat

UNDER CONSTRUCTION

Introduction

Digital Micrograph from Gatan runs on many TEMs for data acquisition. We read and plot such files here.

Import all necessary packages

There are a few setup procedures that need to be followed before any code is written. In this step, we simply load a few python packages that will be necessary in the later steps.

[1]:
%pylab notebook
%gui qt

import sys
from sidpy.io.interface_utils import openfile_dialog
sys.path.insert(0,'../../')
from SciFiReaders import DM3Reader
import SciFiReaders
print('SciFiReaders version: ', SciFiReaders.__version__)
import sidpy
print('sidpy version: ', sidpy.__version__)
Populating the interactive namespace from numpy and matplotlib
WARNING:hyperspy_gui_traitsui:The nbAgg matplotlib backend is not compatible with the traitsui GUI elements. For more information, read http://hyperspy.readthedocs.io/en/stable/user_guide/getting_started.html#possible-warnings-when-importing-hyperspy.
WARNING:hyperspy_gui_traitsui:The traitsui GUI elements are not available.
SciFiReaders version:  0.0.1
sidpy version:  0.0.5

Open a file dialog

Here we select the name of the file to open. We will be using the sidpy interface to do that.

The openfile_dialog will open QT file dialog to select a file.

For examples go to the example_data directory in the original folder of this notebook.

Note.: in some operating systems the window will not show up on top, look in your task bar.

[2]:
file_name = openfile_dialog()
print(file_name)

Catch a bad selection or cancelling of file selection

[4]:
if len(file_name) < 3 or file_name[-4:] != '.dm3':
    print('File not supported')

Read file

We use the ScopeReader to read the file into a sidpy dataset. All metadata (absolutely everything) is saved in the original_metadata attribute of the sidpy Dataset. If the selected file is not a dm3 File you get an IOError.

[5]:
dm3_reader = DM3Reader(file_name)
dataset = dm3_reader.read()

print(dataset)
dataset
sidpy.Dataset of type IMAGE with:
 dask.array<Analog 06, shape=(1600, 1024), dtype=float32, chunksize=(1600, 1024), chunktype=numpy.ndarray>
 data contains: intensity (counts)
 and Dimensions:
x:  distance (counts) of size (1600,)
y:  distance (counts) of size (1024,)
 with metadata: ['image_type']
[5]:
Array Chunk
Bytes 6.55 MB 6.55 MB
Shape (1600, 1024) (1600, 1024)
Count 1 Tasks 1 Chunks
Type float32 numpy.ndarray
1024 1600

Plot File

Only one command is necessary to plot the file.

[6]:
dataset.plot()

DM3 Original Metadata

Digital micrograph stores a lot of metadata in the file and we read all of them and store that information in the original_metadata.

[7]:
dataset.view_original_metadata()
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                        DataType : 23
                        Dimensions :
                                0 : 192
                                1 : 123
                        PixelDepth : 4
                ImageTags :
                Name : Image Of Analog 06
                UniqueID :
                        0 : 703470093
                        1 : 1166020138
                        2 : 1470332098
                        3 : 1364942491
        1 :
                ImageData :
                        Calibrations :
                                Brightness :
                                        Origin : 0.0
                                        Scale : 1.0
                                        Units :
                                Dimension :
                                        0 :
                                                Origin : 0.0
                                                Scale : 0.0625
                                                Units :
                                        1 :
                                                Origin : 0.0
                                                Scale : 0.0625
                                                Units :
                                DisplayCalibratedUnits : True
                        Data : read
                        DataType : 2
                        Dimensions :
                                0 : 1600
                                1 : 1024
                        PixelDepth : 4
                ImageTags :
                        DataBar :
                                Acquisition Date : 4/19/2021
                                Acquisition Time : 12:42:16 PM
                                Exposure Number : 68861
                                System Info : 1.326332413684375e+17
                        DigiScan :
                                Horizontal DS Offset : 0.0
                                Horizontal Image Center : 0.0
                                Horizontal Perpendicular Pixel Step : 0
                                Horizontal Pixel Step : -186
                                Horizontal Spacing : 189.728
                                Vertical DS Offset : 0.0
                                Vertical Image Center : 0.0
                                Vertical Perpendicular Pixel Step : -186
                                Vertical Pixel Step : 0
                                Vertical Spacing : 189.728
                                Zoom factor : 0.57900390625
                        Microscope Info :
                                Cs(mm) : 0.0
                                Emission Current (µA) : 0.0
                                Illumination Mode : STEM
                                Imaging Mode : IMAGING
                                Indicated Magnification : 256.0
                                Items :
                                        0 :
                                                Data Type : 20
                                                Label : Specimen
                                                Tag path : Microscope Info:Specimen
                                                Value : LiMn2O4 NW
                                        1 :
                                                Data Type : 20
                                                Label : Operator
                                                Tag path : Microscope Info:Operator
                                                Value :
                                        2 :
                                                Data Type : 20
                                                Label : Microscope
                                                Tag path : Microscope Info:Microscope
                                                Value : UltraSTEM 100 kV
                                Microscope : UltraSTEM 100 kV
                                Name : None
                                Operation Mode : STEM
                                Operator :
                                Probe Current (nA) : 0.0
                                Probe Size (nm) : 0.0
                                Specimen : LiMn2O4 NW
                                STEM Camera Length : 1.0
                                Voltage : 60000.0
                        SI :
                                Acquisition :
                                        Date : 4/19/2021
                                        End time : 12:44:39 PM
                                        Number of cycles : 1
                                        Pixel time (s) : 0.01
                                        Scan Options :
                                                Scan sub-pixel : True
                                        Spatial Sampling :
                                                Height (pixels) : 64
                                                Width (pixels) : 100
                                        Start time : 12:42:16 PM
                                        Survey Image :
                                                Spectrum Image Rect : [95, 21, 637, 868]
                                                UID :
                                                        0 : 230443211
                                                        1 : 536103321
                                                        2 : 453395375
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                                Scale : 16
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